Journal of Ecophysiology and Occupational Health

P. K. Singh ¹, Sheeba Hussain ¹, Ajay Pratap Singh ¹

Affiliations
1 Department of Zoology, School of Life Sciences, Khandari Campus, Dr. B.R. Ambedkar University, Agra–282 002, IN

Abstract

Arsenic is a known poison of environmental and industrial origin. Prolonged exposure is associated with vascular diseases, skin lesions and cancer. The predominant form of arsenic in the nature is the pentavalent arsenate (AsV), which enters the body mainly via contaminated drinking water. The arsenic contaminated water samples were collected from different areas of Agra district and the arsenic concentration was estimated. Four groups of five albino rats each were administered 0.02 mg/litre arsenic present in drinking water for 7, 14 and 21 days respectively. After stipulated period the liver tissue was isolated for histopathological studies. The body weight, organ weight and their ratio decreased significantly (p<0.001). However, the SGOT, SGPT, ACP and ALP has shown an increase significantly (p<0.001) as compared to control groups. The histopathology of liver shows necrosis, appearance of vacuoles, nuclear degeneration changes after arsenic toxication. However, the alteration in enzymes and histopathological changes are dose dependent in the present investigation.

Keywords

SGPT, SGOT, ACP, ALP, Arsenic, Albino Rat, Hepatopathology.

Full Text

   

P. K. Singh ¹, Jai Singh ¹, Anil Kumar Gupta ¹, Seema Chaudhary ¹

Affiliations
1 Department of Zoology, School of Life Sciences, Khandari Campus, Dr.Bhim Rao Ambedkar University, Agra-282002, IN

Abstract

Arsenic may cause variation in tissues which affect histopathology of reproductive organs. The aim was to investigate the morphological and anatomical changes in structure of testis. In the present study, twenty male albino rats divided into four groups of five rats each. Distilled water and arsenic water were administrated orally to control and experimental groups for 7, 14, and 21 days respectively. The body weight, organ weight, sperm count, sperm motility and sperm sluggish motility were decreased significantly compared with control. The histopathological observations of testes after 7, 14 and 21 days of ground water arsenic treatment of rat showed some predominant change in the structure of testis. There are atrophic changes in testis due to degenerative changes in spermatogenic and leydig cells. The above results confirm the toxic effect of arsenic in testis of albino rats.

Keywords

Arsenic, Organ Weight, Sperm Count, Sperm Motility and Sperm Sluggish.

Full Text